Top 20 cho genome download mới nhất 2022

Tác giả: khủng long www.chogenome.org

Ngày đăng khủng long : 9/5/2021

Đánh giá: 5 ⭐ ( 73158 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 4 ⭐

Tóm tắt: khủng long Bài viết về CHOGenome.org Home. Đang cập nhật…

Khớp với kết quả khủng long tìm kiếm: Search the RefSeq CHO-K1 and Chinese hamster assemblies by gene name, … Welcome to the CHO Genome website! … Proteome · Downloads · Genome Viewer.‎Blast · ‎Resources · ‎CHO Archive: /Genomes… xem ngay

Tác giả: khủng long www.chogenome.org

Ngày đăng khủng long : 27/5/2021

Đánh giá: 2 ⭐ ( 16451 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 1 ⭐

Tóm tắt: khủng long Bài viết về CHOGenome.org Genomic Data Resources. Đang cập nhật…

Khớp với kết quả khủng long tìm kiếm: Links to next generation sequencing raw data in the Sequence Read Archive (SRA) … Click here to access the original CH and CHO genome data files that are …… xem ngay

Tác giả: khủng long www.chogenome.org

Ngày đăng khủng long : 18/5/2021

Đánh giá: 3 ⭐ ( 99368 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 4 ⭐

Tóm tắt: khủng long Bài viết về CHOGenome.org Search CHO-K1 GenBank. Đang cập nhật…

Khớp với kết quả khủng long tìm kiếm: Search CHO-K1 GenBank (2011) assembly by accession number, gene name, symbol, or GO Term: This genome assembly corresponds to the CriGri_1.0 genome assembly …… xem ngay

Tác giả: khủng long www.ncbi.nlm.nih.gov

Ngày đăng khủng long : 3/4/2021

Đánh giá: 3 ⭐ ( 17997 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 1 ⭐

Tóm tắt: khủng long Bài viết về CriGri_1.0 – criGriChoV1 – Genome – Assembly – NCBI. Đang cập nhật…

Khớp với kết quả khủng long tìm kiếm: CriGri_1.0 Genome assembly from the chinese hamster ovary cell line CHO-K1 Organism: Cricetulus griseus (Chinese hamster) Submitter: Beijing Genomics …… xem ngay

Tác giả: khủng long www.ncbi.nlm.nih.gov

Ngày đăng khủng long : 11/5/2021

Đánh giá: 2 ⭐ ( 4443 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 3 ⭐

Tóm tắt: khủng long Accurate and complete genome sequences are essential in biotechnology to facilitate genome‐based cell engineering efforts. The current genome assemblies for Cricetulus griseus, the Chinese hamster, are fragmented and replete with gap sequences …

Khớp với kết quả khủng long tìm kiếm: by O Rupp · 2018 · Cited by 67 Finally, 95% of the sequence gaps were filled, exposing hundreds of cell line‐specific mutations in coding regions of the genome for several CHO cell lines…. xem ngay

Tác giả: khủng long www.nature.com

Ngày đăng khủng long : 18/6/2021

Đánh giá: 2 ⭐ ( 55671 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 3 ⭐

Tóm tắt: khủng long Since 1987, immortalized cells from the ovary of a Chinese hamster have been the workhorse for producing recombinant therapeutics, including monoclonal antibodies, blood factors, hormones, growth factors and enzymes. Xu et al. provide the genome sequence of the ancestral CHO-K1 cell line, which should aid in the optimization of current production cell lines.

Khớp với kết quả khủng long tìm kiếm: by X Xu · 2011 · Cited by 782 Chinese hamster ovary (CHO)–derived cell lines are the preferred host cells for the production of therapeutic proteins…. xem ngay

Tác giả: khủng long www.ensembl.org

Ngày đăng khủng long : 12/8/2021

Đánh giá: 3 ⭐ ( 20463 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 1 ⭐

Tóm tắt: khủng long null

Khớp với kết quả khủng long tìm kiếm: What can I find? Protein-coding and non-coding genes, splice variants, cDNA and protein sequences, non-coding RNAs. More about this genebuild · Download FASTA …

Tác giả: khủng long www.ensembl.org

Ngày đăng khủng long : 27/6/2021

Đánh giá: 2 ⭐ ( 45330 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 3 ⭐

Tóm tắt: khủng long null

Khớp với kết quả khủng long tìm kiếm: Assembly. The Chinese Hamster Ovary cell line (CHO-K1S GS null) assembly, covering more than 90% of the genome is provided by Horizon Discovery and Welcome …Annotation method: Full genebuildAssembly: CHOK1GS_HDv1, INSDC Assembly …Annotation provider: Horizon EagleBase Pairs: 2,358,167,390

Tác giả: khủng long academic.oup.com

Ngày đăng khủng long : 12/8/2021

Đánh giá: 1 ⭐ ( 30224 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 1 ⭐

Tóm tắt: khủng long Bài viết về . Đang cập nhật…

Khớp với kết quả khủng long tìm kiếm: by MP Gerstl · 2017 · Cited by 3 The last decade has seen a surge in published genome-scale … The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.

Tác giả: khủng long www.researchgate.net

Ngày đăng khủng long : 10/6/2021

Đánh giá: 1 ⭐ ( 80381 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 3 ⭐

Tóm tắt: khủng long null

Khớp với kết quả khủng long tìm kiếm: Oct 13, 2021 Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins.

Tác giả: khủng long www.researchgate.net

Ngày đăng khủng long : 17/6/2021

Đánh giá: 1 ⭐ ( 36257 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 2 ⭐

Tóm tắt: khủng long null

Khớp với kết quả khủng long tìm kiếm: Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence …

Tác giả: khủng long bmcgenomics.biomedcentral.com

Ngày đăng khủng long : 13/2/2021

Đánh giá: 3 ⭐ ( 81469 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 5 ⭐

Tóm tắt: khủng long Background The DHFR negative CHO DXB11 cell line (also known as DUX-B11 and DUKX) was historically the first CHO cell line to be used for large scale production of heterologous proteins and is still used for production of a number of complex proteins. Results Here we present the genomic sequence of the CHO DXB11 genome sequenced to a depth of 33x. Overall a significant genomic drift was seen favoring GC → AT point mutations in line with the chemical mutagenesis strategy used for generation of the cell line. The sequencing depth for each gene in the genome revealed distinct peaks at sequencing depths of 0x, 16x, 33x and 49x coverage corresponding to a copy number in the genome of 0, 1, 2 and 3 copies. This indicate that 17% of the genes are haploid revealing a large number of genes which can be knocked out with relative ease. This tendency of haploidy was furthermore shown to be present in eight additional analyzed CHO genomes (15-20% haploidy) but not in the genome of the Chinese hamster. The dhfr gene is confirmed to be haploid in CHO DXB11; transcriptionally active and the remaining allele contains a G410C point mutation causing a Thr137Arg missense mutation. We find ~2.5 million single nucleotide polymorphisms (SNP’s), 44 gene deletions in the CHO DXB11 genome and 9357 SNP’s, which interfere with the coding regions of 3458 genes. Copy number variations for nine CHO genomes were mapped to the chromosomes of the Chinese hamster showing unique signatures for each chromosome. The data indicate that chromosome one and four appear to be more stable over the course of the CHO evolution compared to the other chromosomes thus might presenting the most attractive landing platforms for knock-ins of heterologous genes. Conclusions Our studies reveal an unexpected degree of haploidy in CHO DXB11 and CHO cells in general and highlight the chromosomal changes that have occurred among the CHO cell lines sequenced to date.

Khớp với kết quả khủng long tìm kiếm: by CS Kaas · 2015 · Cited by 69 Results. Here we present the genomic sequence of the CHO DXB11 genome sequenced to a depth of 33x. Overall a significant genomic drift was seen …… xem ngay

Tác giả: khủng long arxiv.org

Ngày đăng khủng long : 9/7/2021

Đánh giá: 4 ⭐ ( 28489 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 1 ⭐

Tóm tắt: khủng long null

Khớp với kết quả khủng long tìm kiếm: by C Monger · 2019 · Cited by 3 Illumina RNA-Seq data utilising the Chinese hamster genome as a reference sequence. The typical stages of a bioinformatics workflow are outlined (Figure 1) …

Tác giả: khủng long link.springer.com

Ngày đăng khủng long : 21/5/2021

Đánh giá: 2 ⭐ ( 53907 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 4 ⭐

Tóm tắt: khủng long Chinese hamster ovary (CHO) cells have frequently been used in biotechnology as a mammalian host cell platform for expressing genes of interest. Previously, we constructed a detailed physical chromosomal map of the CHO DG44 cell line by fluorescence in situ hybridization (FISH) imaging using 303 bacterial artificial chromosome (BAC) clones as hybridization probes (BAC-FISH). BAC-FISH results revealed that the two longest chromosomes were completely paired. However, other chromosomes featured partial deletions or rearrangements. In this study, we determined the end sequences of 303 BAC clones (BAC end sequences), which were used for BAC-FISH probes. Among 606 BAC-end sequences (BESs) (forward and reverse ends), 558 could be determined. We performed a comparison between all determined BESs and mouse genome sequences using NCBI BLAST. Among these 558 BESs, 465 showed high homology to mouse chromosomal sequences. We analyzed the locations of these BACs in chromosomes of the CHO DG44 cell line using a physical chromosomal map. From the obtained results, we investigated the regional similarities among CHO chromosomes (A–T) and mouse chromosomes (1–19 and sex) about 217 BESs (46.7% of 465 high homologous BESs). Twenty-three specific narrow regions in 13 chromosomes of the CHO DG44 cell line showed high homology to mouse chromosomes, but most of other regions did not show significant correlations with the mouse genome. These results contribute to accurate alignments of chromosomes of Chinese hamster and its genome sequence, analysis of chromosomal instability in CHO cells, and the development of target locations for gene and/or genome editing techniques.

Khớp với kết quả khủng long tìm kiếm: by S Kimura · 2018 · Cited by 4 Chinese hamster ovary (CHO) cells have frequently been used in biotechnology as a mammalian host cell platform for expressing genes of …… xem ngay

Tác giả: khủng long www.cell.com

Ngày đăng khủng long : 1/6/2021

Đánh giá: 2 ⭐ ( 81234 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 4 ⭐

Tóm tắt: khủng long null

Khớp với kết quả khủng long tìm kiếm: by FNK Yusufi · 2017 · Cited by 72 over, the recent availability of various CHO cell-line genomes … to the vector sequence and the reference genome (Table S2 and.

Tác giả: khủng long www.mdpi.com

Ngày đăng khủng long : 16/1/2021

Đánh giá: 2 ⭐ ( 84037 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 5 ⭐

Tóm tắt: khủng long null

Khớp với kết quả khủng long tìm kiếm: by FM Wurm · 2017 · Cited by 63 “CHO master sequence” genomes (containing the desired DNAs of interest). Stable cell populations are to be selected for and maintained for …

Tác giả: khủng long hgdownload.soe.ucsc.edu

Ngày đăng khủng long : 29/5/2021

Đánh giá: 1 ⭐ ( 81002 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 1 ⭐

Tóm tắt: khủng long Bài viết về UCSC Genome Browser Downloads. Đang cập nhật…

Khớp với kết quả khủng long tìm kiếm: Human genome. Dec. 2013 (GRCh38/hg38). Genome sequence files and select annotations (2bit, GTF, GC-content, etc)…. xem ngay

Tác giả: khủng long journals.plos.org

Ngày đăng khủng long : 28/1/2021

Đánh giá: 2 ⭐ ( 9053 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 5 ⭐

Tóm tắt: khủng long Chinese hamster ovary (CHO) cell lines represent the most commonly used mammalian expression system for the production of therapeutic proteins. In this context, detailed knowledge of the CHO cell transcriptome might help to improve biotechnological processes conducted by specific cell lines. Nevertheless, very few assembled cDNA sequences of CHO cells were publicly released until recently, which puts a severe limitation on biotechnological research. Two extended annotation systems and web-based tools, one for browsing eukaryotic genomes (GenDBE) and one for viewing eukaryotic transcriptomes (SAMS), were established as the first step towards a publicly usable CHO cell genome/transcriptome analysis platform. This is complemented by the development of a new strategy to assemble the ca. 100 million reads, sequenced from a broad range of diverse transcripts, to a high quality CHO cell transcript set. The cDNA libraries were constructed from different CHO cell lines grown under various culture conditions and sequenced using Roche/454 and Illumina sequencing technologies in addition to sequencing reads from a previous study. Two pipelines to extend and improve the CHO cell line transcripts were established. First, de novo assemblies were carried out with the Trinity and Oases assemblers, using varying k-mer sizes. The resulting contigs were screened for potential CDS using ESTScan. Redundant contigs were filtered out using cd-hit-est. The remaining CDS contigs were re-assembled with CAP3. Second, a reference-based assembly with the Top khủng long Hat/Cufflinks pipeline was performed, using the recently published draft genome sequence of CHO-K1 as reference. Additionally, the de novo contigs were mapped to the reference genome using GMAP and merged with the Cufflinks assembly using the cuffmerge software. With this approach 28,874 transcripts located on 16,492 gene loci could be assembled. Combining the results of both approaches, 65,561 transcripts were identified for CHO cell lines, which could be clustered by sequence identity into 17,598 gene clusters.

Khớp với kết quả khủng long tìm kiếm: by O Rupp · 2014 · Cited by 69 Second, a reference-based assembly with the Top khủng long Hat/Cufflinks pipeline was performed, using the recently published draft genome sequence of …… xem ngay

Tác giả: khủng long www.sciencedirect.com

Ngày đăng khủng long : 16/1/2021

Đánh giá: 3 ⭐ ( 76361 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 5 ⭐

Tóm tắt: khủng long Cell line-specific, genome-scale metabolic models enable rigorous and systematic in silico investigation of cellular metabolism. Such models have rece…

Khớp với kết quả khủng long tìm kiếm: by D Széliová · 2020 · Cited by 19 Moreover, a community-built, genome-scale metabolic model of CHO became available … The following is the supplementary data to this article: Download …… xem ngay

Tác giả: khủng long www.nist.gov

Ngày đăng khủng long : 15/3/2021

Đánh giá: 1 ⭐ ( 23329 lượt đánh giá khủng long )

Đánh giá cao nhất: khủng long 5 ⭐

Đánh giá thấp nhất: khủng long 2 ⭐

Tóm tắt: khủng long The misidentification of cell lines has been a problem in the scientific community for the past 50 years. Methods are now in place to genotype human cell lines using well characterized procedures used in the forensic community based on short tandem repeat (STR) markers; however, methods to identify non-human cell lines are still in the development process. Chinese hamster ovary (CHO) cell lines are heavily used in the manufacturing of recombinant protein therapeutics. To aid in the regulatory and QC stages of development of these therapeutics, an assay to authenticate CHO cells would be beneficial in detecting contamination early on. To date, we have identified nine STR markers specific to CHO DNA and seven markers have been used successfully in a multiplex PCR assay. When improved versions of the CHO genomic sequence are released, we will be able to map the markers to their respective chromosomal locations and develop additional markers.

Khớp với kết quả khủng long tìm kiếm: Sep 9, 2013 When improved versions of the CHO genomic sequence are released, we will be able to map the markers to their respective chromosomal …… xem ngay